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Lonza
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ImmunoTools
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Meso Scale Diagnostics LLC
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PeproTech
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STEMCELL Technologies Inc
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Wellstat Therapeutics Corporation
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GENTAUR Inc
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inTEST Corporation
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KOENEN GmbH
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Image Search Results
Journal: PLoS ONE
Article Title: Directed Differentiation of Embryonic Stem Cells Using a Bead-Based Combinatorial Screening Method
doi: 10.1371/journal.pone.0104301
Figure Lengend Snippet: (a) Dot plots illustrating flow cytometry analysis of cells obtained after maturation in IL7 supplemented MethoCult for 2 weeks, showing the appearance of cells co-expressing B220/CD43 and B220/CD19; (b) Histogram showing the proportions of different cell populations present in MethoCult or OP9 derived cultures, measured by flow cytometry analysis. Blue bars represent cells taken off beads on D15 (prior to maturation), red bars represent cell isolated on D15 and cultured for a further 2 weeks on OP9 feeder layers and green bars represent cells isolated on D15 and cultured in MethoCult for 2 weeks. The experiment was carried out twice and in duplicate the bars show the average percentage of cells, the error bars show the standard deviation. There is a statistically significant difference between the cells taken from the beads at D15 and those cultured on MethoCult for 2 weeks for markers CD43 and CD19 *p<0.05.
Article Snippet: To test this, we plated cells generated by Cluster A protocols, either at d9 or d15 of differentiation, into MethoCult semisolid medium supplemented with
Techniques: Flow Cytometry, Expressing, Derivative Assay, Isolation, Cell Culture, Standard Deviation